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WashU MRC-WUCCI Partnership for 3D X-Ray Imaging

The Washington University Musculoskeletal Research Center (MRC) and Center for Cellular Imaging (WUCCI) have partnered to acquire and operate a “3D X-ray microscope” for high-resolution imaging of a wide variety of biological samples (from bone to brain to kidney to fat) as well as synthetic biomaterials. This resource is now available to all members of the Washington University research community.

Application (Word format)

Bone image (1.8 um resolution)
reveals microvascular canals (red,
green) and osteocyte lacunae
(blue). (ZEISS, MRC & Silva lab)
Mouse embryo – multiple
tissues stained with KI3
(James Fitzpatrick/WUCCI &
Eghtesady Lab)
Woven biomaterial scaffold
seeded with adult stem cells
(bright). (Matt Joens/WUCCI
& Guilak Lab)

 

Mouse Kidney – stained with
Os / UA (Matt Joens/WUCCI
& Humphreys Lab)
Mouse Kidney – stained with KI3 –
showing cancer metasteses in green
(Matt Joens/WUCCI & Weilbaecher Lab)

 

The ZEISS Xradia 520 Versa 3D X-ray microscope (XRM) is capable of sub-micron resolution, high-contrast x-ray computed tomography (CT) imaging. The system has specifications on par with other “nanoCT” systems, and in addition has dual-energy and phase-contrast capabilities to extend the range of applications beyond radiodense materials. Its purchase was funded by the NIH Office of Research Infrastructure Programs through a High-End Instrumentation Grant (1 S10 OD021694-01) awarded to Dr. Matthew Silva, Department of Orthopaedic Surgery and Musculoskeletal Research Center. The system is operated by Dr. James Fitzpatrick, Department of Neurology and Center for Cellular Imaging, as part of a partnership between the MRC and WUCCI. It is located at the WUCCI (4515 McKinley Research Building, Washington University School of Medicine). For information, please contact James Fitzpatrick (fitzp[at]wustl.edu) or Matthew Silva (silvam[at]wustl.edu), or visit wucci.wustl.edu or musculoskeletalcore.wustl.edu.

Applications of 3D X-Ray Microscope

The XRM system is for ex vivo imaging of samples from < 1 mm to ~10 cm in size. There is a wide-range of sample geometries and imaging resolutions (voxel size) that are possible, and imaging settings (energy, filters, projections, integration time, etc.) are all under user control, allowing for great flexibility. The applications that we have already demonstrated with the XRM are shown below. This is an illustration of possible applications – we are excited to try new applications so please contact us if you have a project or idea.

1. Cortical Bone – porosity & microcracks

(Left) 2D slice of cortical bone from rabbit ulna imaged
at 0.99 um resolution reveals vascular and cellular
(osteocyte) microporosities. (Right) 3D vascular porosity
image from this sample. (Dan Leib, MRC & Silva Lab)
2D slice of rat ulna imaged at 0.85um
reveals microcrack after fatigue
loading. (ZEISS, MRC & Silva Lab)

 

XRM scan of mouse tibia imaged at 1.8 um reveals vascular and cellular microporosity. (Left) 3D view
of cortex with porosity of sub-region shown in red. (Middle) Porosity color coded by volume – smaller
osteocyte lacunae are blue; vascular pores are green or red. (Right) Histogram reveals distribution of
porosity size. (ZEISS, MRC & Silva Lab)

2. Bone marrow adipose tissue

2D slice through mouse tibia imaged at 2.2
um reveals individual lipid droplets in the
bone marrow (Osmium contrast; Dan
Leib/MRC & Scheller/Craft Lab)

3. Fracture Callus and Vascularity

3D reconstructions of mouse femur w/
fracture callus. (Left) Mineralized
sample with low-density callus and
vessels. (Right) Demineralized sample
showing only vasculature. (Microfil
contrast; James Fitzpatrick/WUCCI,
Dan Leib/MRC & Silva Lab)

4. Whole Joints

Sagittal and coronal slices through
mouse knee joint revealing
mineralized and non-mineralized
tissues, incl. subchondral bone (blue
arrows), articular cartilage (red
arrows), ligaments, joint capsule,
meniscus and muscle. (Scanned at 4.5
um voxel size with PTA contrast;
ZEISS, MRC & Silva Lab)
Sagittal slice through rat elbow revealing
humero-radial and humero-ulnar articulations.
Anterior joint capsule is seen between
humeral condyle and muscle. (Scanned at
4.9 um voxel size with PMA
contrast; Dan Leib/MRC & Lake Lab)

5. Vertebra & Intervertebral Disc

2D slice of mouse tail vertebra-disc-vertebra reveals
details of intervertebral disc (IVD) structure, including
outer annulus, inner nucleus and endplates. (Scanned
at 1.9 um voxel size with PMA contrast; Dan
Leib/MRC & Tang Lab)span>