Available Mouse Strains

Skeletal-specific Cre lines currently available

*UPDATED October 2018*

 Cre-Mice

Strain Name

Application

WU P.I.

Original P.I.

Reference

OSX-cre OB deleter Abu-Amer Civitelli  
LysM-cre macrophage deleter Abu-Amer Jax  
Col2cre CHC/OB delete Abu-Amer Civitelli  
DMP1-cre osteocyte Deleter Abu-Amer L. Bonewald  
CD11b-cre macrophage delete Abu-Amer  J-P Vacher  
Dermo1-Cre Targeting mesenchyme broadly Ornitz Ornitz Yu et al., Dev. 2003
Col2CreER(T) Tamoxifen-controllable targeting of chondrocytes Sandell Susan Mackem Nakamura et al., Dev Dyn 2006
LysM-Cre Targeting myeloid cells Teitelbaum Jackson Labs Croke et al., J Cell Sci 2011
Cathepsin K Cre(T) Targeting osteoclasts Teitelbaum Shigiaki Kato Nakamura et al., Cell 2007
Adiponectin-Cre Targeting adipocytes Teitelbaum  Jackson Labs  
Alb-Cre Targeting hepatocytes Teitelbaum  Jackson Labs  
 Integrin beta3 -/- important for osteoclasts and platelets function Teitelbaum     
Cdh5-Cre-ERT2  (Vecad-Cre-ERT2) Tm inducible targeting vascular endothelial cells Silva Ralf Adams    MTA reqd
Osx-Cre-ERT2 Tm inducible, targeting of (pre)osteoblasts Silva Kronenberg    MTA reqd Maes et al. 2007, 2010
UBC-cre;ERT2  Tm inducible, global cre
Silva Jax  (Stock #007001)
Dmp1-Cre-ERT2 Tm inducible targeting mature osteoblasts & osteocytes Silva Divieti-Pajevic (From Robling) Permission reqd  
LysM-Cre Targeting immune cell lineage
Silva Jax  (Stock #004781)  
OC-Cre Targeting mature osteoblasts
Silva Jax #019509  Clemens
LysM-Cre Target cells of Myeloid origin Faccio     Jackson Labs   
Agc1-CreERT2 Tm inducible targeting of chondrocytes O'Keefe Benoit deCrombrugghe Genesis, 2009 47:805-814
Osterix-CreERT2 Tm-inducible targeting of pre-osteoblasts McAlinden H. Kronenberg (MTA req) Maes et al, 2007, 2010
Agc1CreERT2 Tm inducible targeting of chondrocytes McAlinden  B. deCromrugghe Genesis, 2009  47:805-814
Prx1-Cre Targeting mesenchymal progenitor cells that form cells of the limbs and cranium  McAlinden  C. Tabin Logan, et al. Genesis, 2002; 33(2):77-80 PMID-12112875
Available from Jackson labs (https://www.jax.org/strain/005584)
Advillin-Cre-ERT2 Tamoxifen-inducible deletor for DRG and sensory neurons  Scheller Jackson Labs (stock # 026516)  
 

Floxed Mice

Strain Name

Application

WU P.I.

Original P.I.

Reference

IKK2-floxed

conditional IKK2 deletion

Abu-Amer M. Pasparakis  
NEMO-fl

conditional NEMO del

Abu-Amer M. Pasparakis  
Vegf flox Conditional deletion of Vegf Silva Genentech (from Olsen lab), MTA Reqd Gerber et al, 1999, Development
Wnt1 flox Conditional deletion of Wnt1 Silva Brendan Lee & Kyu-Sang Joeng (Baylor) Permission reqd Joeng et al, 2017, JCI
Wnt7b flox Conditional deletion of Wnt7b Silva Jax #008467  
Wls flox Conditional deletion of Wntless Silva Jax #012888  
Cxcr2 flox Conditional deletion of Cxcr2 Silva Jax #024638  
Il6-flox Conditional deletion of Interleukin-6 Silva    
ASXL1 flox/flox epigenetic regulation gene Teitelbaum ECOMMA  
PGC1b flox/flox important for mitochondria biogenesis Teitelbaum Schilling  
DTA stop-flox useful for deletion of specific cell type Teitelbaum Harris  
Floxed S1P
(Site-1 protease; Mbtps1)
Delete S1P in tissue of choice using a desired tissue-specific Cre Sandell Jay Horton (UT Southwestern Med. Center) Yang et al., 2001, PNAS
β-catenin constitutively active β-catenin mutant allele in which exon 3 is flanked by loxP sequences Faccio    Harada et al., 1999.
Lrp5 A214V (floxed allele) Lrp6 mutation causing high bone mass Civitelli Matthew Warman Nat Med. 2011 Jun;17(6):684-91.
Connexin43 flox Conditional and germline deletion of connexin43 Civitelli Klaus Willecke Mol Biol Cell. 2011 Apr 15;22(8):1240-51.
Connexin45 flox Conditional and germline deletion of connexin45 Civitelli Klaus Willecke J Neurosci 2005 25: 566–576
Connexin 43 G138R mutation (floxed allele) Human occulodigital dysplasia mutation Civitelli Klaus Willecke Hum Mol Genet. 2008 Feb 15;17(4):539-54
N cadherin flx Conditional deletion of N cadherin Civitelli Glenn Radice J Cell Sci 2010 123, 2640-2648.
Smad4 flx Conditional deletion of Smad4 Civitelli Chu-Xia Deng Genesis 2002 32, 80-81.
Floxed Piezo 1 Conditional deletion of Piezo 1 Guilak Ardem Patapoutian (available from Jax)  
Floxed Piezo 2 Conditional deletion of Piezo 2 Guilak Ardem Patapoutian (available from Jax)  
Double-floxed Piezo 1 & 2 Conditional deletion of both Piezo 1 & 2 Guilak Guilak  
Dnmt3b flox Conditional deletion of Dnmt3b O'Keefe Li J Biol Chem, 2005 280:17986-91
FoxO1 flox Conditional deletion of FoxO1 O'Keefe
Jax (024756)  
Igf1r flox Conditional deletion of Igf1r O'Keefe Jax (012251)  
Nlrp3 flox Conditional NLRP3 activation Mbalaviele Hal Hoffman https://www.ncbi.nlm.nih.gov/pubmed/22558291
Parp-1 flox Conditional PARP-1 deletion Mbalaviele M. Hottiger (MTA req’d)  
Igr1r Conditional deletion of Igf1 receptor McAlinden Jackson Lab Available from Jackson lab (https://www.jax.org/strain/012251)
miR-181a/b-1 Conditional deletion of miR-181a/b-1 cluster McAlinden Chen Available from Jackson lab
https://www.jax.org/strain/025872
NIK delta TRAF3 (NT3) Cre-directed, targeted activation of NIK Veis Marc Schmidt-Supprian Sasaki, Y., Calado, D. P., Derudder, E., Zhang, B., Shimizu, Y., Mackay, F., et al. (2008). NIK overexpression amplifies, whereas ablation of its TRAF3-binding domain replaces BAFF:BAFF-R-mediated survival signals in B cells. Proc Natl Acad Sci USA, 105(31), 10883–10888. http://doi.org/10.1073/pnas.0805186105
IKK1 floxed conditional deletion of IKK1 Veis Yingling Hu Liu, B., Xia, X., Zhu, F., Park, E., Carbajal, S., Kiguchi, K., et al. (2008). IKKα Is Required to Maintain Skin Homeostasis and Prevent Skin Cancer. Cancer Cell, 14(3), 212–225. http://doi.org/10.1016/j.ccr.2008.07.017
Mfn2 floxed conditional deletion of Mfn2 Veis David C Chan Chen H; McCaffery JM; Chan DC. 2007. Mitochondrial fusion protects against neurodegeneration in the cerebellum. Cell 130(3):548-62PubMed: 17693261MGI: J:132329
Floxed ATRAID (in C57BL6 background) Conditional deletion of ATRAID Peterson Peterson (also available from Jax, strain # 026576)
 

KO Mice

Strain Name

Application

WU P.I.

Original P.I.

Reference

PLCγ2-/- (phospholipase C gamma 2 -/-) Innate immune cell defects, reduced osteoclast numbers and function, increased bone mass. Faccio Dr. T. Ilhe  
PKC delta -/- Osteoblast defects during embryogenesis, defective OC postnatally, high bone mass Faccio Nakayama KI, Nakayama K  
Lyn -/- Lyn is a Src family member.  Lyn -/- mice have increased innate immune cell activation and numbers, enhanced osteoclasts; no basal bone phenotype, and reduced BV/TV after RANKL challenge Faccio Dr. C. Lowell  
Perforin KO mice defective T cells, bone phenotype not reported Faccio Jackson Lab  
SOST null (and congenic WT mice) Germline deletion of sclerostin Civitelli Amgen (MTA req'd) J Bone Miner Res. 2008 Jun;23(6):860-9
Connexin43 null Conditional and germline deletion of connexin43 Civitelli Klaus Willecke Mol Biol Cell. 2011 Apr 15;22(8):1240-51.
Cadherin 11 null Germline deletion of Cadherin 11 Civitelli Akira Kudo J Cell Sci 2010 123, 2640-2648.
IL6 KO Interleukin-6 knockout Silva Jax 002650  
Nlrp3-/- Germline Nlrp3 null mice Mbalaviele Jax# 021302 https://www.ncbi.nlm.nih.gov/pubmed/28747793
Nlrc4-/- Germline Nlrc4 null mice Mbalaviele Genentech (MTA req’d) https://www.ncbi.nlm.nih.gov/pubmed/20603313
Gsdmd-/- Germline Gsdmd null mice Mbalaviele Genentech (MTA req’d) https://www.ncbi.nlm.nih.gov/pubmed/26375259
Il-1r-/- Germline Il-1 receptor null mice Mbalaviele Jax #003018 https://www.ncbi.nlm.nih.gov/pubmed/28687790
Caspase-1-/- Germline Casp1 null mice Mbalaviele TD Kanneganti (MTA req’d)  
Caspase-11-/- Germline Casp11 null mice Mbalaviele Jax #024698  
Caspase-1;11-/- Germline Casp1;11 null mice Mbalaviele Jax #004947  
Parp-1-/- Germline Parp1 null mice Mbalaviele Jax #002779  
RAGE-KO Global deletion of RAGE Tang Schmidt (NYU), Bierhaus (Heidelberg) Liliensiek B et al. J Clin Invest 113: 1641–1650, 2004.
β-catenin KO loxP sites introduced 5′ of exon 2 and 3′ of exon 6 of β-catenin to achieve cell-specific inactivation of β-catenin Faccio   Brault et al, 2001.
 

Reporter Mice

Strain Name

Application

WU P.I.

Original P.I.

Reference

Ai9 Cre reporter  (TdTom) Silva Jax #007905  
Tcf/Lef-GFP Wnt reporter Silva Jax 013752  
mTmG Reporter Two color fluorescent Cre recombinase reporter mice Scheller Jackson labs (stock # 007676)  
TRAP-Red (OC reporter mice) Td-Tomato osteoclast reporter mice Veis Masaru Ishii Kikuta, J. et al. Dynamic visualization of RANKL and Th17-mediated osteoclast function. J. Clin. Invest. 123, 866–873 (2013).
NFkb-eGFP/LUC Reporter for cellular localization of NFkb activation and quantification Tang Jax (#027529) Everhart MB et al. 2006. J Immunol 176(8):4995-5005
 

Miscellaneous Mice

Strain Name

Application

WU P.I.

Original P.I.

Reference

Akita Ins2 heterozygous; diabetic mouse Silva Jax 003548  
3.6Col1a1-tk Upon exposure to ganciclovir replicating osteoblasts undergo apoptosis Silva Jilka & O'Brien Jilka et al, 2009
Dnmt3b-tg Conditional overexpression of Dnmt3b O'Keefe O'Keefe  
Igf1-tg Conditional overexpression ofIgf1 O'Keefe O'Keefe  
FoxO1-tg Conditional overexpression of FoxO1 O'Keefe Jax (016262)  
LEPR-db/db To model phases I to III diabetes type II and obesity. Manifest morbid obesity, chornic hyperglycemia, pancreatic beta cell atrophy and become hypoinsulinemic Tang Jax (#000642) Gueorguiev M et al. 2001. Pituitary 4(1-2):79-86
Thy1-YFP-16 Express yellow fluorescent protein at high levels in motor and sensory neurons, as well as in subsets of central neurons. Tang Jax (#003709) Feng G et al. 2000. Neuron 28(1):41-51
LG/J cartilage healer strain Rai Cheverud  
SM/J cartilage non-healer strain Rai Cheverud  
ROSA26-miR-181a/b-1-knock-in Conditional over-expression of miR-181a/b-1 cluster McAlinden McAlinden (in house; still being characterized)
ROSA26-Igf2-knock-in Conditional over-expression of Igf2 McAlinden McAlinden (in house; still being characterized)
DTA mice Express diphtheria Toxin alpha chain gene (DTA) after Cre-mediated removal of a floxed neo/STOP cassette Scheller Jackson labs (stock # 009669)