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Available Mouse Strains

Skeletal-specific Cre lines currently available

 

 Cre-Mice

Strain Name

Application

WU P.I.

Original P.I.

Reference

OSX-cre

OB deleter

Abu-Amer Civitelli  
LysM-cre

macrophage deleter

Abu-Amer Jax  
Col2cre

CHC/OB delete

Abu-Amer Civitelli  
DMP1-cre

osteocyte Deleter

Abu-Amer L. Bonewald  
CD11b-cre

macrophage delete

 Abu-Amer  J-P Vacher  

Col2-Cre3

 

Target both chondrocyte and osteoblast lineages

Long

 

Col1-Cre

osteoblasts               

Long

Kronenberg (permission req'd)

 

GLi1-CreERT2

Hh-responsive cells

Long

Joyner    (available from JAX)

 

Prx1-Cre        

Mesenchymal progenitors in limbs and skull

Long

Tabin     (available from JAX)

Osx-Cre

Osteoblast precursors, dox off system

Long

McMahon(available from JAX)

Dmp1-Cre

Osteocytes and other cells

 
Long     Feng (MTA req'd)

Dermo1-Cre

Targeting mesenchyme broadly

Ornitz

Ornitz

Yu et al., Dev. 2003

Col2CreER(T)

Tamoxifen-controllable targeting of chondrocytes

Sandell

Susan Mackem

Nakamura et al., Dev Dyn 2006

LysM-Cre

 

Teitelbaum

 

 

Cathepsin K Cre(T)

 

Teitelbaum

Shigiaki Kato

 

Scleraxis Cre

Targeting tendon-specific knockout

Thomopoulos

Ronen Schweitzer

Dev Cell. 2009 Dec;17(6):861-73.

VE-Cadherin-Cre
(Cdh5-Cre)

Target vascular endothelial cells

Silva

Jax
(Stock #006137)

 

Osx-Cre-ERT2

Tm inducible, targeting of (pre)osteoblasts

Silva

Kronenberg
(via Civitelli lab)

Maes et al. 2007, 2010

UBC-cre;ERT2
(Ubiquitin C)

Tm inducible, global cre

Silva

Jax
(Stock #007001)

 

LysM-Cre

Targeting myeloid cells

Teitelbaum

Jackson Labs

Croke et al., J Cell Sci 2011.

LysM-Cre Target cells of Myeloid origin Faccio     Jackson Labs   

10kb DMP1-Cre

Osteocyte/odontoblast gene deletion

Civitelli     Lynda Bonewald (MTA req'd)

J Dent Res. 2007 Apr;86(4):320-5

 

Floxed Mice

Strain Name

Application

WU P.I.

Original P.I.

Reference

IKK2-floxed

conditional IKK2 deletion

Abu-Amer M. Pasparakis  
NEMO-fl

conditional NEMO del

Abu-Amer M. Pasparakis  

Scleraxis GFP FLX

Deletion of the transcription factor scleraxis
(necessary for tendon development)

Thomopoulos

Ronen Schweitzer

Development. 2007 Jul;134(14):2697-708. Dev Dyn. 2007 Jun;236(6):1677-82.

Bmp2flox

Conditional deletion of Bmp2

Silva

Vicky Rosen

Tsuji et al. 2006

Vegf flox Conditional deletion of Vegf Silva

Genentech, Needs MTA

Gerber et al, 1999, Development

CathepsinK-Cre

Targeting osteoclasts

Teitelbaum

 

Nakamura et al., Cell 2007

Floxed S1P

(Site-1 protease; Mbtps1)     

Delete S1P in tissue of choice using a desired tissue-specific Cre Sandell  Jay Horton (UT Southwestern Med. Center)  Yang et al., 2001, PNAS
β-catenin KO loxP sites introduced 5′ of exon 2 and 3′ of exon 6 of β-catenin to achieve cell-specific inactivation of β-catenin Faccio    Brault et al, 2001.
β-catenin constitutively active β-catenin mutant allele in which exon 3 is flanked by loxP sequences Faccio    Harada et al., 1999.

Lrp5 A214V (floxed allele)

Lrp6 mutation causing high bone mass

Civitelli Matthew Warman

Nat Med. 2011 Jun;17(6):684-91.

Connexin43 flox

Conditional and germline deletion of connexin43

Civitelli Klaus Willecke

Mol Biol Cell. 2011 Apr 15;22(8):1240-51.

Connexin45 flox Conditional and germline deletion of connexin45 Civitelli Klaus Willecke 

J Neurosci 2005 25: 566576.

Connexin 43 G138R mutation (floxed allele)

Human occulodigital dysplasia mutation

Civitelli Klaus Willecke Hum Mol Genet. 2008 Feb 15;17(4):539-54

N cadherin flx

Conditional deletion of N cadherin

Civitelli

Glenn Radice

J Cell Sci 2010 123, 2640-2648.

Smad4 flx

Conditional deletion of Smad4

Civitelli

Chu-Xia Deng

Genesis 2002 32, 80-81.

Lrp5 f/f          LRP5 floxed allele     Long    

 Williams (MTA req'd)

 
Smo f/f

Smo floxed allele

Long  McMahon (available from Jax)  
Notch2 f/f Notch2 floxed allele Long  (available from Jax)  
 

KO Mice

Strain Name

Application

WU P.I.

Original P.I.

Reference

Cho mice

Collagen XI alpha 1 null.
Model for studying
chondrodysplasia and
collagen assembly in cartilage.

McAlinden

Robert Seegmiller

http://www.ncbi.nlm.nih.gov/pubmed/7859283; http://www.ncbi.nlm.nih.gov/pubmed/17683922

Col2a1-A

Homozygote mice express
only the embryonic (IIA)
form of Col2a1. Mice
are viable and can be
used to study mechanisms
of matrix assembly in
cartilage and bone.

McAlinden

McAlinden

http://www.ncbi.nlm.nih.gov/pubmed/22248926

Col2a1-C

Homozygote mice express increased
levels of embryonic Col2a1 IIA
collagen due to a mutation
in the IIC splice site in
Col2a1 exon 2. Mice are
viable and can be used
to study mechanisms of
matrix assembly in
cartilage and bone.

McAlinden

McAlinden

http://www.ncbi.nlm.nih.gov/pubmed/24735995

 

PLCγ2-/- (phospholipase C gamma 2 -/-) Innate immune cell defects, reduced osteoclast numbers and function, increased bone mass. Faccio Dr. T. Ilhe  
PKC delta -/- Osteoblast defects during embryogenesis, defective OC postnatally, high bone mass Faccio Nakayama KI, Nakayama K  
Lyn -/- Lyn is a Src family member.  Lyn -/- mice have increased innate immune cell activation and numbers, enhanced osteoclasts; no basal bone phenotype, and reduced BV/TV after RANKL challenge Faccio Dr. C. Lowell  
Perforin KO mice defective T cells, bone phenotype not reported Faccio         Jackson Lab   

SOST null (and congenic WT mice)

Germline deletion of sclerostin

Civitelli

Amgen (MTA req'd)

J Bone Miner Res. 2008 Jun;23(6):860-9

Connexin43 null

Conditional and germline deletion of connexin43

Civitelli

Klaus Willecke

Mol Biol Cell. 2011 Apr 15;22(8):1240-51.

Connexin45 null

Conditional and germline deletion of connexin45 Civitelli

Klaus Willecke

J Neurosci 2005 25: 566576.

Cadherin 11 null

Germline deletion of Cadherin 11

Civitelli

Akira Kudo

J Cell Sci 2010 123, 2640-2648.

Ihh+/- Ihh knockout         Long McMahon (available from JAX)  
 

Reporter Mice

Strain Name

Application

WU P.I.

Original P.I.

Reference

TOPGAL tg

Wnt reporter

Silva

Jax
(Stock #004623)

 

mTmG

Cre reporter
(red off; green on)

Silva

Jax
(Stock #007576)

 

Ai9

Cre reporter
(red on)

Silva

Jax
(Stock #007905)

 

 

Miscellaneous Mice

Strain Name

Application

WU P.I.

Original P.I.

Reference

R26-ΔNGli2

Rosa26 knockin of constitutively active Gli2, activated by Cre

Long Long

 

R26-Smo* Rosa26 knockin of constitutively active Smo, activated by Cre Long McMahon (available from JAX)  
R26-Wnt7b

Rosa26 knockin of Wnt7b, activated by Cre

Long Long  
PPARg-tTA

tTA expressed from endogenous PPARg locus

Long

Barak (available from Jax)

 
Runx2-rtTA rtTA expressed from Runx2 BAC Long Long  
R26-mT/mG Rosa26 knockin of mT/mG cassette, GFP induced by Cre Long Luo (available from Jax)  
3.6Col1a1-tk Upon exposure to ganciclovir replicating osteoblasts undergo apoptosis Silva Jilka Jilka et al, 2009