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Musculoskeletal Histology and Morphometry Core
(In Situ Molecular Analysis)

Director

Deborah Novack
Bone Histology
and Histomorphometry
314-454-8472
novack@wustl.edu

Technical Staff

Histology – Crystal Idleburg HT(ASCP)
Sr. Research Tech/Histotech
314-747-6034
idleburgc@wudosis.wustl.edu 

Samantha Coleman
Research Tech II
747-2753
scoleman22@wustl.edu


Billing Administrator

Kami McGhee
Orthopaedic Surgery
314-747-5993
314-454-5900 - fax
kjm@wustl.edu

Overview

Form and function are intimately interrelated. However, the histological analyses of our target tissues (bone, cartilage, tendon, ligament, muscle) provide specialized challenges not often encountered when conducting these techniques on non-skeletal tissues. For example, due to the extensive extracellular matrix, these tissues can be very difficult to prepare and section, and bone presents a particular challenge due to mineralization. A specialized knowledge of the stages of development, and limb and joint morphology is necessary for the analysis of skeletal tissues. In addition to morphology, the localization of proteins and mRNA in mouse models of musculoskeletal diseases is a vital component in the analysis of the molecular mechanism of protein function, the response to injury, and disease. The overall objective of the Histology and Morphometry Core is to provide histological services for the identification and analysis of molecular phenotypes of our target tissues, bone, cartilage, muscle, tendon and ligament, in developing and adult mice and other model organisms, as well as patient samples.

The services include:

  1. Preparation of sections for all musculoskeletal tissues (bone, cartilage, tendon, ligament, muscle), including paraffin, methylmethacrylate, and frozen (OCT) embedding and specific tissue orientations, including a variety of specialized stains.
  2. Provision of specialized histology techniques, including nondecalcified frozen sections with tape transfer for fluorescent label visualization, immunostaining, in situ hybridization or laser capture microdissection. Training and protocols can also be provided.
  3. Bone histomorphometry, training and access to equipment.
  4. Consultation on histological techniques and choice of reagents.

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Go to the Calendar and select the Bioquant tab to schedule.

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Cllick links to view Dr. Novack's presentations

Bone Histomorphometry 101
Bone Histomorphometry Guidelines
What's New in Histomorphometry (2014)

 


 

 

 

Citing the grant in publications:


“Washington University Musculoskeletal Research Center (NIH P30 AR057235)”